Studies on Fermentation and Immobilization of Bioluminescent Bacteria

Neeraj, Katiyar (2011) Studies on Fermentation and Immobilization of Bioluminescent Bacteria. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	The fermentation of the bioluminescent bacteria Photobacterium leiognathiwas done for the production of biomass in a 10 litre stirred tank fermenter which was initially used for the immobilization studies. The wet weight of the bacterial pellet was measured to be 12g.Further, studies on the immobilization of P. leiognathi was carried out in matrices viz. Sodium alginate, carrageenan, agar, Chitosan, gelatin and polyacrylamide for their application as whole cell bioluminescent biosensor. The stable and prolonged luminescence response was observed in the bacteria immobilized in sodium alginate, carrageenan and agar matrix. It was found that the luminescence efficiency of the cells immobilized in sodium alginate, carrageenan and agar matrix were 92%, 78% and 30%.These immobilized bioluminescent beads can be applied as whole cell biosensor for the detection of environmental pollutants, toxins, xenobiotics and heavy metal contaminations in a very short period of time.
Uncontrolled Keywords:	Photobacterium leiognathi, fermentation, environmental pollutants
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 Jul 2011 09:19
Last Modified:	28 Dec 2011 10:25
URI:	http://ir.cftri.res.in/id/eprint/10236

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