Endogenous profiles of indoleamines: serotonin and melatonin in different tissues of Coffea canephora P ex Fr. as analyzed by HPLC and LC-MS-ESI. fermentation: parameter optimization and application for N-acetyl chitooligosaccharides preparation from chitin.

Endogenous indoleamine profiles in various ex
vitro and in vitro tissues of commercially important Coffea
canephora were analyzed by using a high performance liquid
chromatography and further confirmed with electrospray
ionization mass spectrometry. High content of serotonin
(SER) (98.54 ± 5 lg/g) and melatonin (MEL) (115.25 ± 6
lg/g) were found in freshly harvested seeds of C. canephora
followed by zygotic embryo (65.25 ± 4 and 96.54 ± 5 lg/g
freshweight) and endosperm(34.08 ± 2 and 51.08 ± 4 lg/g
fresh weight) of ripened fruits. Similarly endogenous pools
of SER and MEL were moderate in in vitro tissues of
C. canephora, i.e. callus (25.85 ± 2 and 75.74 ± 4), somatic
embryos (31.88 ± 2 and 19.30 ±2 lg/g fresh weight) and in
vitro regenerated plant stalk (15.78 ± 1 and 38.25 ± 3 lg/g
fresh weight), respectively. In view of significant levels of
both SER and MEL in various tissues and beans of Coffea,
further investigations on their physiological role needs to be
investigated.