Isolation and identification of pelargonidin 3-glucoside in mangosteen pericarp.

In the present study, we have identified pelargonidin 3-glucoside, along with two known anthocyanin;
cyanidin 3-sophoroside and cyanidin 3-glucoside, from acidified, methanolic extract of mangosteen pericarp.
The compounds were separated by preparative HPLC after purification by partition against ethyl
acetate and Amberlite XAD-7. The structures of the compounds were confirmed by high performance
liquid chromatography (HPLC), UV–Vis absorption spectra, high-resolution electrospray mass spectrometry
and 1D, 2D nuclear magnetic resonance (NMR) spectroscopy. This new pigment family adds to the
growing body of data supporting the use of natural colourants in food. Cyanidin 3-sophoroside was
the major anthocyanin detected in large amount (76.1%), followed by cyanidin 3-glucoside (13.4%) and
pelargonidin 3-glucoside (6.2%).