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Immunoassay for the detection of lead ions in environmental water samples.
Mandappa, I. M. and Ranjini, A. and Haware, D. J. and Manjunath, M. N. and Manonmani, H. K. (2012) Immunoassay for the detection of lead ions in environmental water samples. International Journal of Environmental Analytical Chemistry, 92 (3). pp. 334-343.
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International Journal of Environmental Analytical ChemistryVolume 92, Issue 3, March 2012, pages 334-343.pdf - Published Version Restricted to Registered users only Download (209kB) | Request a copy |
Abstract
A rapid, simple, and reliable competitive immunoassay was developed for measurement of lead ions Pb(II) in environmental samples. Avian antibodies were produced against Pb(II). Since lead ions are too small to elicit an immune response, the metal was coupled to protein carrier Bovine serum albumin (BSA) using a bifunctional chelator 1-(4-isothiocyanobenzyl) ethylenediamine N,N,N0, N0-tetra acetic acid (ITCBE). Poultry birds (layers) were immunised with this Pb(II)–ITCBE–BSA immunoconjugate and the avian antibodies (IgY) isolated from egg yolk recognised Pb(II)-ITCBE complexes as capture reagent and a Pb(II)–ITCBE conjugate of Alkaline phosphatase as an enzyme label. Antibody reaction was optimised for different concentrations of antigen and antibody dilutions. Cross reactivity with other metals were below 1% in competitive ELISA. The IC50 value of this avian antibody was 0.19 mgmL�1. The detection range and the detection limit were 0.02–1000 mgmL�1and 0.2 mgmL�1, respectively.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | avian antibodies (IgY); cross reactivity; detection of lead ions; ELISA; environmental samples; specificity |
| Subjects: | 600 Technology > 01 Medical sciences > 17 Toxicology 600 Technology > 08 Food technology > 17 Water activity |
| Divisions: | Fermentation Technology and Bioengineering Food Safety Analytical Quality Control Lab |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 23 Feb 2012 10:04 |
| Last Modified: | 18 Nov 2016 11:15 |
| URI: | http://ir.cftri.res.in/id/eprint/10665 |
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