Extraction of bromelain from pineapple core and purification by RME and precipitation methods

Chaurasiya, Ram Saran and Umesh Hebbar, H. (2013) Extraction of bromelain from pineapple core and purification by RME and precipitation methods. Separation and Purification Technology, 111. pp. 90-97. ISSN 1383-5866

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Abstract

Demand for bromelain is increasing because of its application in various fields like medical, food and cosmetics. While bromelain extracted from pineapple stem is commercially known, presence of bromelain is reported also in core, peel and crown of pineapple fruit. Core, which is slightly harder in texture, is one of the major wastes generated (15% of the fruit) by the pineapple processing industry. The present work mainly focuses on optimization of conditions for extraction of bromelain from pineapple (Ananas comosus L.) core and purification of bromelain by reverse micellar extraction (RME) and precipitation techniques. Extraction conditions like type of buffer, pH, temperature and strength of buffer on bromelain activity and protein content were standardized. Storage stability study of the extract under refrigerated condition (5–8 °C) showed decrease in bromelain activity with duration. Mineral analysis of extract showed the presence of calcium, sodium, potassium, and magnesium in the extract. RME, one of the promising liquid–liquid extraction techniques was employed for the primary purification of bromelain from pineapple core. Selective separation of bromelain (excluding polyphenol oxidase (PPO) and peroxidase (POD)) with 3.96-fold purification and activity recovery of 78.90% was achieved with this method. Acetone and ammonium salt precipitation methods resulted in 4.90 and 3.07-fold purification under optimized conditions. The studies indicate that extraction followed by RME or precipitation method could be employed for separation and purification of bromelain from extract obtained from pineapple core.

Item Type:	Article
Additional Information:	Unmapped bibliographic data: PY - 2013/6/25/ [EPrints field already has value set] JO - Separation and Purification Technology [Field not mapped to EPrints]
Uncontrolled Keywords:	Bromelain, Pineapple core, Precipitation, Purification, RME
Subjects:	600 Technology > 08 Food technology > 01 Analysis 600 Technology > 08 Food technology > 24 Fruits
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Sep 2016 11:09
Last Modified:	22 Sep 2016 11:09
URI:	http://ir.cftri.res.in/id/eprint/11211

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