Permeabilized probiotic Lactobacillus plantarum as a source of b-galactosidase for the synthesis of prebiotic galactooligosaccharides.

Gobinath, D. and Prapulla, S. G. (2014) Permeabilized probiotic Lactobacillus plantarum as a source of b-galactosidase for the synthesis of prebiotic galactooligosaccharides. Biotechnology Letters, 36. pp. 153-157.

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Abstract

Permeabilized probiotic Lactobacillus plantarum was used as a source of b-galactosidase for the synthesis of galactooligosaccharides (GOS) from lactose. b-galactosidase activity was highest when galactose (1,724 Miller Units) was used as a carbon source compared to lactose, sucrose or glucose at 37 �C, 18 h. Permeabilized cells had the highest transgalactosylation activity resulting in 34 % (w/w) GOS synthesis from 40 % (w/v) lactose at 50 �C over 12 h. HPLC revealed that the GOS were composed of 13 % disaccharides (non-lactose), 17 % trisaccharides and 4 % tetrasaccharides that were further confirmed by ESI–MS.

Item Type:	Article
Uncontrolled Keywords:	Galactooligosaccharides Lactobacillus plantarum Permeabilized cells Prebiotics Probiotics
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	21 Jan 2014 09:08
Last Modified:	21 Jan 2014 09:08
URI:	http://ir.cftri.res.in/id/eprint/11317

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