Extracellular chitin deacetylase production in solid state fermentation by native soil isolates of Penicillium monoverticillium and Fusarium oxysporum.

Extracellular chitin deacetylase production by
native soil isolates of Penicillium monoverticillium CFR 2
and Fusarium oxysporum CFR 8 in solid state fermentation
(SSF) using commercial wheat bran (CWB) and shrimp
processing by-products (SPP) as solid substrate has been
studied. P. monoverticillium produced maximum chitin
deacetylase activity of 547.7±45 and 390.2±31 units/g initial
dry substrate (U/g IDS) at 96 h of incubation in CWB
and SPP media, respectively. While, F. oxysporum produced
maximum chitin deacetylase activity of 306.4±22 U/g IDS
at 72 h of incubation in CWB medium and 220.1±20 U/g
IDS at 120 h of incubation in SPP medium. Along with
chitin deacetylase, P. monoverticillium and F. oxysporum
produced other chitin degrading enzymes such as endochitinase
and β-N-acetylhexosaminidase. P. monoverticillium
produced maximum activity (U/g IDS) of endochitinase
4.6±0.20 at 120 h incubation and β-N-acetylhexosaminidase
82.6±03 at 120 h incubation in CWB medium.
While, F. oxysporum produced maximum activity (U/g IDS)
of endo-chitinase 7.8±0.20 at 144 h incubation and β-Nacetylhexosaminidase
38.3±02 at 120 h incubation in CWB
medium. Production of extracellular chitin deacetylase by P.
monoverticillium CFR 2 and F. oxysporum CFR 8 in SSF is
being reported for the first time.