Cost-Effective Endo-Mannanase from Bacillus Sp. Cfr1601 and its Application in Generation of Oligosaccharides from Guar Gum and as Detergent Additive

Praveen Kumar, Srivastava and Mukesh, Kapoor (2014) Cost-Effective Endo-Mannanase from Bacillus Sp. Cfr1601 and its Application in Generation of Oligosaccharides from Guar Gum and as Detergent Additive. Preparative Biochemistry and Biotechnology, 44. pp. 392-417.

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Abstract

The indigenous bacteria Bacillus sp. CFR1601 produced significant levels of endomannanase when grown on agro-wastes, namely, green gram husk and sunflower oil cake (25.6 IU=mL), used as sole carbon and nitrogen sources, respectively. Under immobilized cell system, synthetic supports (polyurethane foam, scotch brite, polyester; up to 33.2 IU=mL) were found marginally superior as compared to natural supports (cotton and silk; up to 28.2 IU=mL) for endo-mannanase production. Cooperative interactions between L-lysine HCl (0.3% w=v), Tween 60 (0.3% v=v), and sunflower oil cake (3.0% w=v) in central composite design response surface methodology ameliorated (1.61-fold) endo-mannanase titers to 48.0 IU=mL. Partially purified endo-mannanase was tested for its ability to produce oligosaccharides from guar gum. These oligosaccharides were tested in vitro for their ability to promote growth of Lactobacillus plantarum MTCC 5422 and Lactobacillus salivarius CHS 1E. Results indicated that low-molecular-weight degraded products from guar gum were (1) able to support the growth of tested strains [increased O.D600nm up to 2.3-fold and decrease in pH (<6.3) due to production of short chain fatty acid (SCFA)] when used as sole carbon source; and (2) after purification and analysis by electron spray ionization–mass spectrometry (ESI-MS) were found to be composed of mainly disaccharide and tetrasaccharide. The compatibility of endo-mannanase with various detergents together with wash performance test confirmed its potential applicability for laundry industry.

Item Type:	Article
Uncontrolled Keywords:	Bacillus sp., detergents, endo-mannanase, oligosaccharides, response surface methodology, whole cell immobilization
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 May 2016 12:22
Last Modified:	19 May 2016 12:22
URI:	http://ir.cftri.res.in/id/eprint/12188

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