Optimization of culture conditions for bacterial cellulose production from Gluconacetobacter hansenii UAC09

Fermentation conditions for the production of
bacterial cellulose (BC) by a newly isolated Gluconacetobacter
hansenii UAC09 were optimized. The effect of
various carbon and nitrogen sources on production of BC
was determined. The addition of ethanol (1.5%) and acetic
acid (1.0%) enhanced BC production by more than three
times compared to standard Hestrin and Schramm medium.
The strain was able to produce maximum BC (7.40 g l−1)
with 4% glucose, 8% corn steep liquor (CSL) at 27±1°C
and a pH of 5.5 under stationary conditions. The BC films
produced under various conditions were tested for their
structural similarity using Fourier transform-infra red
analysis and were found to be similar to each other
irrespective of medium composition. Scanning electron
microscopy images of BC revealed a porous structure with
extensively interwoven microfibrils.