Eugenol and n-3 Rich Garden Cress Seed Oil as Modulators of Platelet Aggregation and Eicosanoids in Wistar Albino Rats.

Eugenol, the active principle of clove oil is a potent antioxidant and a well known anti-inflammatory
agent. In an earlier study, we have reported the in vitro inhibition of human platelet aggregation and LTC4 formation
in rat PMNL cells. In the present study, an attempt has been made to monitor the in vivo and in vitro modulation
of platelet aggregation and eicosanoids (TXB2, LTC4) by eugenol and a-linolenic acid (ALNA) rich garden
cress seed oil in adult Wistar rats. Feeding of Garden cress oil (GCO) supplemented diet to rats for 8 weeks moderately
inhibited platelet aggregation induced by ADP and arachidonic acid (AA) at 14 and 7% respectively and also
TXB2 levels at 10 and 32% in lung and spleen tissues. Oral feeding of eugenol at 40 mg/kg body weight for one
week to GCO fed rats showed 20-30% inhibition of ADP and AA induced platelet aggregation and also TXB2
levels by ~ 50% in spleen. In an in vitro study, eugenol at 15.0 and 45.0