Preparation of Docosahexaenoic Acid (DHA) Nanoemulsion and Investigation of its Stability.

Docosahexaeneic acid (C22:6 DRA) is derived from marine algae. In-vitro,
live animal and human trials suggested that DHA has beneficial effects on human
health, including prevention and curing of cognitive disorders, cardiac and
circulatory disorders and inflammatory disorders, etc. Nanoencapsulation can
protect the risk of oxidation of polyunsaturated DHA from the surrounding
environment such as higher temperature, air, pH and light. In this study, DHA
nanoemulsion was prepared by oil in water (O/W) emulsion method using different
types of emulsifiers, respectively. The stability of emulsion was analyzed by storage
condition, colour and phase separation. The emulsion droplet size distribution
and structure were analyzed by trinocular microscope. The optimum stability of
nano-emulsion was achieved by the above method and size of the nanoemulsion
was in the range ef300-900 nm. Hence, this nano DHA emulsion can be used for
food and pharmaceutical applications.