Recovery of Proteins from Rohu Processing Waste Using pH Shift Method: Characterization of Isolates.

Yield and physicochemical characteristics of protein isolates recovered from
rohu processing waste by acid- and alkali-aided protein solubilization methods
were studied. pH 2.0 from acidic side and pH 13.0 from alkaline side
resulted in maximum protein solubility, whereas minimum solubility was
observed at pH 5.5. Except for pH 13.0, theoretical protein recovery was
more than the actual recovery after centrifugation. Maximum protein recovery
after the first centrifugation cycle was observed for pH 2.0 and pH 13.0, and
the maximum protein recovery during the second centrifugation cycle was
observed at precipitation pH 5.5 in both acidic and alkaline methods. Acidic
and alkaline methods resulted in a total process recovery of 31.81% ± 0.001%
and 31.11% ± 0.01%, respectively. Isolates prepared by acidic method had
more whiteness and high foaming capacity, whereas isolates prepared using
alkaline methods had less whiteness, tough texture, and high foaming stability.
Nomicrobial counts were detected in any of the isolates. Acid and alkaline
methods described in this study can be used for the recovery of proteins from
rohu processing waste with good yields and functionality.