LC–ESI–MS based characterisation of isoflavones in soybean (Glycine max (L.) Merr.) from India.

A total of twenty-one soybean varieties were
screened for their morphological characteristics followed
by isoflavone content analysis by HPLC. The total isoflavone
(TI) content was found within a wide range of
140.9–1048.6 lg/g of soy in different varieties. The highest
isoflavone content was found in MAUS-2 followed by
DS_2613 and lowest in Karunae (140.9 lg/g of soy).
Various isoflavone forms were identified by LC–ESI? MS.
Significant differences in the isoflavone content were
observed for all the aglycones and their glucoside conjugates
as well as total daidzein, total genistein, total glycitein,
and TI. A positive correlation between TI content and
growth stages was found during the progression of seed
development. An increase of 5.4-fold and 5.3-fold of TI
concentration was observed for JS 335 and MAUS-2
respectively, from early to green mature (R5–R8) stage of
bean development.