Formation of Bowman–Birk inhibitors during the germination of horsegram (Dolichos biflorus)

Kumar, Pradeep and Sreerama, Yadahalli N. and Gowda, Lalitha R. (2002) Formation of Bowman–Birk inhibitors during the germination of horsegram (Dolichos biflorus). Phytochemistry, 60. pp. 581-588.

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Abstract

Three Bowman–Birk type inhibitors (HGGI-I, II and III), which appear in the cotyledons of 120 h germinated horsegram (Dolichos biflorus) seeds have been purified to homogeneity by size-exclusion chromatography and ion-exchange chromatography. HGGI-I, HGGI-II and HGGI-III differ from each other and from the dormant seed inhibitors in amino acid composition, molecular size and charge. The amino-terminal sequence analyses indicate that these inhibitors are derived from the isoinhibitors of the dormant seed by a limited proteolysis and not by de novo synthesis. These inhibitors differ from each other at their amino-terminus. HGGI-II identical to HGGI-I except for the loss of a single amino-terminal aspartyl residue, where as HGGI-III shows the loss of a pentapeptide. All the three inhibitors are potent competitive inhibitors of trypsin and chymotrypsin. The dissociation constants (Kis) for trypsin inhibition indicate that amino-terminal tail of the inhibitors play a role in trypsin binding probably through electrostatic interaction.

Item Type:	Article
Uncontrolled Keywords:	Dolichos biflorus; Horsegram seeds; Germination; Bowman–Birk inhibitors; Amino-terminal proteolysis
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry 600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions:	Protein Chemistry and Technology Lipid Science and Traditional Foods
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	24 Sep 2007 11:08
Last Modified:	28 Dec 2011 09:30
URI:	http://ir.cftri.res.in/id/eprint/1426

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