Dephytinising efficacy of wheat phytase in enhancing the bioaccessibility of minerals in high phytate foods.

Poorvisha, R. and Uma, T. N. and Jyothi Lakshmi, A. (2020) Dephytinising efficacy of wheat phytase in enhancing the bioaccessibility of minerals in high phytate foods. Journal of Food Measurement and Characterization.

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Abstract

Iron defciency anemia and zinc defciency are major public health problems in developing world which are attributable to the poor bioavailability due to the presence of phytic acid in plant foods. Dephytinisation by enzymatic application is identifed to be ideal approach, however the commercial availability of phytases from food sources is scarce. This prompted us to undertake the investigation on the dephytinisation of the high phytate food matrices by utilising the indigenous phytase of wheat. Attempts were made to enhance the phytase activity by various approaches. Phytase activity of wheat was increased by fvefold on sprouting (96 h). The native phytase of wheat activated by incubation at optimum conditions of pH and temperature has shown an increase in the activity as a function of time. Isolation and partial purifcation of phytase from sprouted wheat further increased the activity by 1.5 fold. Thermostability of phytase was enhanced by additives such as sodium citrate, calcium chloride and magnesium sulphate. Addition of phytase activated wheat four to soya four enhanced the bioaccessible iron by 1.7 fold. Partially purifed phytase enhanced the bioaccessible iron by 1.9 fold and zinc by 2.4 fold in defatted soya four refecting dephytinisation. Investigation on complete purifcation of phytase are warranted.

Item Type:	Article
Uncontrolled Keywords:	Wheat phytase · Defatted soya four · Partial purifcation · Bioaccessibility · Dephytinisation
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 21 Cereals > 04 Wheat
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Oct 2020 08:44
Last Modified:	05 Oct 2020 08:44
URI:	http://ir.cftri.res.in/id/eprint/14498

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