Lactobacillus fermentum MCC2759 and MCC2760 Alleviate Infammation and Intestinal Function in High‑Fat Diet‑Fed and Streptozotocin‑Induced Diabetic Rats.

Ann Catherine, Archer and Muthukumar, S. P. and Prakash, M. Halami (2021) Lactobacillus fermentum MCC2759 and MCC2760 Alleviate Infammation and Intestinal Function in High‑Fat Diet‑Fed and Streptozotocin‑Induced Diabetic Rats. Probiotics and Antimicrobial Proteins.

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Abstract

The growing incidence of type 2 diabetes and obesity has become a worldwide crisis with increased socio-economic burden. Changes in lifestyle and food habits resulting in dysbiosis of the gut microbiota and low-grade infammation are linked to the rising incidence. The aim of this study was to investigate the efects of potential probiotic Lactobacillus fermentum MCC2759 and MCC2760 on intestinal markers of infammation using a high-fat diet (HFD)-fed model and a streptozotocin (STZ)-induced diabetic model. Lact. fermentum administration showed improved oral glucose tolerance compared with the model controls of HFD (AUC 1518) and STZ (628.8). Plasma insulin levels improved in the Lact. fermentum treated groups of HFD+MCC2759 (129±4.24 pmol/L) and HFD+MCC2760 (151.5±9.19 pmol/L) in HFD study, while in STZ diabetic study, the insulin levels were normalized with Lact. fermentum administration, for D+MCC2759 (120.5±7.77) and D+MCC2760 (138±5.65 pmol/L) groups. The results showed reduction in infammatory tone in liver, muscle, and adipose tissues of rats in both models with stimulation of anti-infammatory IL-10 by real-time quantitative polymerase chain reaction. Additionally, the potential probiotic cultures also displayed normalization of markers related to intestinal barrier integrity (ZO-1), TLR-4 receptor, and insulin sensitivity (GLUT-4, GLP-1, adiponectin). Thus, the results suggest that Lact. fermentum could act as potential probiotic for lifestyle-related disorders such as obesity, diabetes, and metabolic syndrome as both prophylactic and adjunct therapies.

Item Type:	Article
Uncontrolled Keywords:	Probiotics Lactobacillus spp. IL-10 GLP-1 Streptozotocin (STZ)-induced diabetes
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology 600 Technology > 01 Medical sciences > 04 Diabetes Mellitus
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	09 Mar 2021 11:19
Last Modified:	09 Mar 2021 11:19
URI:	http://ir.cftri.res.in/id/eprint/14806

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