Direct osmosis for the concentration of bromelain from pineapple

Chethan, D. S. and Hrishikesh, A. and Rajeshwari, V. and Shyama, Chandran (2007) Direct osmosis for the concentration of bromelain from pineapple. [Student Project Report]

Abstract

Item Type:	Student Project Report
Additional Information:	Generally biomolecules are concentrated by anti-dialysis /ultrafiltration process. The main drawbacks of these existing processes are: Difficulty in scaling up in case of antidialysis and membrane fouling and concentration polarization effects in case of ultrafiltration process. In the present work direct osmosis, relatively a newer process is used as an alternative method for the concentration of biomolecules. Bromelain, a proteolytic enzyme present in pineapple is used as a enzyme. During direct osmosis process, the effect of various process parameters such as sodium chloride (osmotic agent) concentration (2 to 6 m), flow rate (30 to 100 ml/min) and enzyme extract (feed) flow rate (30 to 100 ml/min) on transmembrane flux and bromelain activity is examined. Increase in sodium chloride solution concentration from 2-6m resulted in an increase in transmembrane flux from 3.21 to 4.5 l/m2h and bromelain activity from 1999 to 2587 CDU/ml. The transmembrane flux increased from 1.23 to 4.50 l/m2h with an increase in osmotic agent flow rate due to decrease in concentration polarization effect.
Uncontrolled Keywords:	Bromelain pineapple direct osmosis Biomolecules
Subjects:	600 Technology > 08 Food technology > 24 Fruits 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Nov 2007 11:43
Last Modified:	03 Sep 2018 09:50
URI:	http://ir.cftri.res.in/id/eprint/1518

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