Studies on the partial characterization of extracted glycosaminoglycans from fish waste and its potentiality in modulating obesity through in-vitro and in-vivo.

Geetha, V. and Moumita, Das and Mehrdad, Zarei and Nanishankar, V. Harohally and Suresh Kumar, G. (2022) Studies on the partial characterization of extracted glycosaminoglycans from fish waste and its potentiality in modulating obesity through in-vitro and in-vivo. Glycoconjugate Journal.

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Abstract

Glycosaminoglycans (GAGs) are bioactive polysaccharides or glycoconjugates found in the fsh waste having signifcant health impacts. In the present study it has been attempted to extract GAGs from mackerel fsh waste through chemical and enzymatic methods. Further, the extracted GAGs (e-GAGs) were analyzed for their composition (uronic acid, total sugar & sulfate), chemical characterization was carried out through techniques of scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) & Proton NMR. Further, probable major GAGs present was identi�fed by enzymatic digestion. The biological potential of the extracted glycoconjugate was assessed further through in-vitro and in-vivo studies. In-vitro biological activity showed good lipase inhibition (IC50, 2.6 mg/mL) and bile acid binding properties (dose-dependent). Lipid accumulation lowered in the e-GAGs diferentiated 3T3L1 preadipocyte cells have also been observed. The high fat fed animal (in-vivo) study showed ameliorative efect via reducing blood sugar∼1.28↓, lipid profle↓, plasma insulin∼3.5↓, improved glucose tolerance, and homeostatic model assessment for insulin resistance (HOMA-IR, ∼3.0↓). Furthermore, elimination of bile acid (BA) due to GAG-BA binding properties resultant in removal of elevated fecal triglyceride and cholesterol suggesting its lipid lowering activity. Regulation of various proteins linked to carbohydrate and lipid metabolism including fatty acid synthase (FAS), low density lipoproteins receptor (LDL-R), 7α-hydroxylase, glucose transporter-4 (GLUT4) and Peroxisome proliferator- activated receptor gamma (PPAR-γ) were signifcant (p<0.05) with e-GAGs treatment when compared to HFD group. Thus, the e-GAGs showed potential hypo�lipidemic activity through elimination of bile acid binding property together with regulating the specifc protein related to obesity and its associated complications.

Item Type:	Article
Uncontrolled Keywords:	Fish waste · Glycosaminoglycans · Anti-obesity · C57BL/6 mice · 3T3L1 pre-adipocytes · Bile acid binding
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry 600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish
Divisions:	Dept. of Biochemistry Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	25 Jan 2023 04:31
Last Modified:	25 Jan 2023 04:31
URI:	http://ir.cftri.res.in/id/eprint/15867

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