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Biomass production and bioactive secondary metabolites of bioreactor grown callus suspension cultures of Swallow root (Decalepis hamiltonii Wight & Arn.)
Umashankar, K. and Giridhar, P. (2022) Biomass production and bioactive secondary metabolites of bioreactor grown callus suspension cultures of Swallow root (Decalepis hamiltonii Wight & Arn.). Journal of Emerging Technologies and Innovative Research, 9 (3). pp. 153-158.
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Abstract
In the present study establishment of in vitro callus cultures of D. hamiltonii on MS medium followed by its biomass augmentation trials in Erlenmeyer conical flask and stirred tank bioreactor (STB) was investigated. The callus suspension culture grown in 150mL Erlenmeyer flask showed 9.1 g FW per 50 ml culture (182 gL-1 FW of biomass) and 0.097±0.21mg 100g-1 DW of 2H4MB in 4 weeks of growth. The methanol extract of the callus showed 13.1±0.2 mg 100g�1 DW and 0.46±0.03 mg 100g-1 DW of TPC and TFC respectively. Whereas in callus culture grown in 2 lit medium in STB, 33.5 g FW of biomass was recorded and 0.024± 0.007 mg 100g�1 DW 2H4MB upon 7 days growth. The methanol extract of fermenter-grown-callus showed 2.13± 0.39 mg 100g�1 DW and 0.12± 0.4 mg 100g-1 DW of TPC and TFC respectively. The study infers D. hamiltonii callus culture in a bioreactor can be an alternative method for producing vanillin isomer in large-scale production and further optimization of culture conditions in this regard is required.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Callus suspension cultures, 2H4MB, Stirred tank bioreactor, Swallow root |
| Subjects: | 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture 600 Technology > 08 Food technology > 23 Vegetables > Swallow root |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 15 May 2023 10:32 |
| Last Modified: | 15 May 2023 10:32 |
| URI: | http://ir.cftri.res.in/id/eprint/16339 |
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