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Flash chromatographic isolation of garcinol and isogarcinol from Garcinia indica Choisy (kokum) fruit and evaluation of their potential antibiofilm activity
Prachi Aparichita, Patra and Drishya, Palakkandi and Anil Kumar, Krishnegowda and Monisha, Vijayarengan and Sarma, Mutturi and Shivakumar, Linganna and Subban, Nagarajan (2025) Flash chromatographic isolation of garcinol and isogarcinol from Garcinia indica Choisy (kokum) fruit and evaluation of their potential antibiofilm activity. Microbial Pathogenesis, 198. pp. 1-12.
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Abstract
The present study describes the isolation and separation of isogarcinol and garcinol from kokum fruit by flash chromatography using water (containing 0.1 % formic acid) and methanol mixed with acetonitrile (1:1) with UV detection at 254 nm and was characterised by HR-MS and NMR studies. These were further subjected to anti-microbial studies on Staphylococcus aureus FR1722 and Listeria monocytogenes Scott A by agar diffusion assay and broth microdilution method wherein the MIC (Minimum Inhibitory Concentration) of garcinol and isogarcinol were, 20 μg/mL and 50 μg/mL for S. aureus FR1722 and 50 μg/mL and 100 μg/mL for L. monocytogenes Scott A respectively. Further, studies on the control of biofilm growth were tested using MTT (3-(4,5-dimethyl-2-thia-zolyl)-2,5-diphenyl-2H tetrazolium bromide) assay followed by Crystal Violet (CV) assay, which showed up to 80 % inhibition. In addition, the biofilm eradication as evaluated by bright field microscopy and the metabolic activity were also carried out against these bacterial strains and the experimental details are presented.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Garcinol, Isogarcinol, Flash chromatography, Antibiofilm, Listeria monocytogenes, Staphylococcus aureus |
| Subjects: | 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 18 Phytochemistry 600 Technology > 08 Food technology > 24 Fruits |
| Divisions: | Plantation Products Spices and Flavour Technology |
| Depositing User: | Somashekar K S |
| Date Deposited: | 05 Mar 2025 11:22 |
| Last Modified: | 05 Mar 2025 11:22 |
| URI: | http://ir.cftri.res.in/id/eprint/19196 |
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