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Agrobacterium‑mediated genetic transformation of chia (Salvia hispanica L.), a rich source of omega‑3 fatty acid
Anirudh, N. and Haritha, P. K. and Sreedhar, R. V. (2025) Agrobacterium‑mediated genetic transformation of chia (Salvia hispanica L.), a rich source of omega‑3 fatty acid. Journal of Transgenic Research, 34 (31). pp. 1-16.
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Abstract
Chia (Salvia hispanica L.), a nutrition- ally valuable crop, is the richest source of α-linolenic acid, a key omega-3 fatty acid. Despite its nutritional benefits, a stable genetic transformation method for chia is not available. This study presents a sonication- assisted Agrobacterium-mediated transformation pro- tocol optimized for high-efficiency transformation of chia seedlings. Key parameters including bacterial cell density, acetosyringone concentration, sonica- tion duration, vacuum infiltration, and infection time were optimized. Results demonstrated that an opti- cal density at 600 nm (OD600) of 0.5, acetosyrin- gone concentration of 100 μM, 20 min of sonication, 10 min of vacuum infiltration, and 60 min of infec- tion significantly enhanced transformation efficiency and GUS expression. This optimized protocol was validated through Polymerase Chain Reaction and β-glucuronidase (GUS) assay in transformed plants. Our findings establish a robust and reliable transfor- mation protocol, paving the way for future genetic engineering efforts aimed at enhancing the nutritional and agronomic traits of chia.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Salvia hispanica L. · Agrobacterium tumefaciens · GUS · Genetic transformation · Transgenic plant |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry 600 Technology > 08 Food technology > 22 Legumes-Pulses |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Somashekar K S |
| Date Deposited: | 07 Oct 2025 06:12 |
| Last Modified: | 07 Oct 2025 06:12 |
| URI: | http://ir.cftri.res.in/id/eprint/19889 |
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