Scanning electron microscopy of chemically and enzymatically treated black gram (Phaseolus mungo) and ragi (Eleusine coracana) starch granules

Tharanathan, R. N. and Ramadas Bhat, U. (1988) Scanning electron microscopy of chemically and enzymatically treated black gram (Phaseolus mungo) and ragi (Eleusine coracana) starch granules. Starch/Staerke, 40 (10). pp. 378-382. ISSN 0038-9056

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Abstract

Amylolysis of chemically-treated starch granules of black gram and ragi was followed colorimetrically and by scanning electron microscopy (SEM). % hydrolysis values indicated that oxidation with periodate resulted in considerable resistance, whereas urea treatment resulted in granule susceptibility towards in vitro amylolysis. The action of alpha-amylase and/or glucoamylase on periodate oxidized black gram starch showed on SEM enlargement of the granules and hollow craters, whereas similar treatment on oxidized ragi starch resulted in folded, distored and deformed granules. Treatment with 8M urea resulted in total loss of characteristic granular shape and formation of so-called amylopectin `sacs'. Synergistic action of alpha-amylase and glucoamylase on urea-treated starches virtually collapsed the granules, forming innumerable `flat' ribbon-like fragments.

Item Type:	Article
Uncontrolled Keywords:	STARCHES-SPECIFIC; black gram starch, amylolysis of oxidized; ragi starch, amylolysis of oxidized; LEGUMES-; MILLETS-; AMYLASES-; OXIDATION-
Subjects:	600 Technology > 08 Food technology > 21 Cereals 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 30 Starch Chemistry 600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 Jul 2008 04:25
Last Modified:	30 Apr 2012 09:22
URI:	http://ir.cftri.res.in/id/eprint/2005

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