Evaluation of the antihypertensive effect of sialoglycoprotein hydrolysate from Labeo rohita roes using Wistar rats

Anushma, Venmarath and Muthukumar, S. P. and Tanaji, G. Kudre (2025) Evaluation of the antihypertensive effect of sialoglycoprotein hydrolysate from Labeo rohita roes using Wistar rats. Food Bioscience, 73. pp. 1-12.

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Abstract

Fish roes, rich in sialoglycoproteins, are often discarded, raising environmental concerns. The current study aimed to evaluate the antihypertensive effects of sialoglycoprotein hydrolysate produced from Labeo rohita roes using Wistar rats. SGPH exhibited moderate but significant ACE-inhibitory activity (74.70 ± 0.13 %), DPPH radical scavenging activity (37.85 ± 1.08 %), ABTS radical scavenging activity (47.15 ± 1.60 %), and FRAP activity (27.13 ± 0.03 μM Trolox equivalents/g). The ACE-inhibition kinetics and gastrointestinal (GI) di- gestibility of SGPH demonstrated that SGPH was a competitive inhibitor and relatively stable in simulated GI conditions. SGPH revealed a moderate but significant antihypertensive effect on hypertensive (2K1C) Wistar rats in a dose-dependent manner by reducing systolic and diastolic blood pressure (SBP and DBP) and kidney ACE activity (P < 0.05) after 4 weeks of treatment. Reduction of SBP, DBP, and kidney ACE activity ranged from 140 ±2–128 ± 2 mmHg, 100±3–82 ± 3 mmHg, and 0.94 ± 0.01–0.61 ± 0.02 μM HA/min/mg, respectively. Notably, SGPH elevated the superoxide dismutase and catalase activities in 2K1C rats (P < 0.05), irrespective of SGPH dose, which could augment additional cardio-protection by minimizing oxidative stress. SGPH-treated rat groups displayed urine, haematology and serum biochemistry values in the normal range, indicating no adverse effects of SGPH. Further, histopathological evaluation elucidated that there was no incidence of organ abnor- mality in SGPH-treated rats, asserting that SGPH precluded vital organ injuries. Therefore, SGPH could be a promising antihypertensive ingredient that can be deployed in functional foods/nutraceuticals development for combating hypertension and its related diseases.

Item Type:	Article
Uncontrolled Keywords:	Labeo rohita roes, Sialoglycoprotein hydrolysate, ACE-Inhibitory activity, Antioxidant activity, Bioactive sialoglycopeptides, Wistar rats, In vivo antihypertensive effect
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry
Divisions:	Dept. of Biochemistry Meat Fish and Poultry Technology
Depositing User:	Somashekar K S
Date Deposited:	13 Nov 2025 10:57
Last Modified:	13 Nov 2025 10:57
URI:	http://ir.cftri.res.in/id/eprint/20092

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