The lasso peptide produced by Bacillus licheniformis MCC 2514 demonstrates efficacy in treating in-vivo Salmonella Typhimurium infection

Ishrat Jahan, Peerzade and Muthukumar Serva, Peddha and Prakash, M. Halami (2024) The lasso peptide produced by Bacillus licheniformis MCC 2514 demonstrates efficacy in treating in-vivo Salmonella Typhimurium infection. International Journal of Biological Macromolecules, 281. pp. 1-14.

PDF 1-s2.0-S0141813024072799-main.pdf - Published Version Restricted to Registered users only Download (8MB) | Request a copy

Abstract

Salmonella, a significant pathogen transmitted through food, presents a substantial threat to public health. The issue of antibiotic misuse is causing a quest for alternative replacements. An antimicrobial peptide, predicted as lasso peptide 2514 (LP-2514), derived from the probiotic bacteria Bacillus licheniformis MCC 2514, has demon- strated effectiveness against various foodborne pathogens including Salmonella. This study aims to assess the efficacy of this peptide in vivo. Mice infected with Salmonella Typhimurium received daily oral administration of LP-2514 (30 mg/kg/day) for 2 weeks until the symptoms subsided. After the treatment, biochemical and his- topathological parameters were examined. LP-2514 treated mice demonstrated reduced infection, as evidenced by a 5-fold decrease in aspartate aminotransferase concentration and a 10-fold decrease in alanine amino- transferase concentration in plasma. Nitric oxide generation was decreased by 61.23 %, C-reactive protein by 75.9 %, and numerous antioxidant enzymes were elevated to suppress the infection. Increased expression of the anti-inflammatory marker Interleukin-10 (IL-10) by 43-fold was observed in treated mice, while untreated mice displayed elevated expression of pro-inflammatory cytokines indicating the severity of infection. Hence, LP-2514 successfully alleviated the disease symptoms caused by S. Typhimurium, thus exhibiting as a potential replace- ment for antibiotics or food-grade preservatives.

Item Type:	Article
Uncontrolled Keywords:	Lasso-peptide, Antimicrobial peptide, Salmonella Typhimurium
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 25 Peptide Chemistry 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions:	Dept. of Biochemistry Food Microbiology
Depositing User:	Somashekar K S
Date Deposited:	09 Dec 2025 05:21
Last Modified:	09 Dec 2025 05:21
URI:	http://ir.cftri.res.in/id/eprint/20160

Actions (login required)

View Item