Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.) seed husk and its enzyme inhibitory potential associated with hyperglycaemia

Uma Maheshwari, Srinivasa and Madeneni Madhava, Naidu (2021) Selective extraction of galactomannan from fenugreek (Trigonella foenum-graecum L.) seed husk and its enzyme inhibitory potential associated with hyperglycaemia. Journal of the Science of Food and Agriculture, 101. pp. 4751-4759.

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Abstract

BACKGROUND: Fenugreek seeds host various bioactive compounds, and galactomannan (GM) is a significant soluble fibre. In this study, selective extraction is adapted to extract fenugreek seed GM to improvise the yield recovery. The seeds are fraction- ated, separated and classified as husk and cotyledons. Comparative studies have been performed to evaluate the crude and pure GM yield between different groups such as the whole seed, and the classified fractions. Characterization is done using Fou- rier transform infrared, differential scanning calorimetry, scanning electron microscopy, monosaccharide composition and optical density, and the structure is elucidated through nuclear magnetic resonance. The GM obtained through extraction is used to study its enzyme inhibitory property associated with hyperglycaemia. RESULTS: GM yield extracted from the husk is highly significant compared to other groups. Crude GM and pure GM yield was 2 and 3.25 times higher than that obtained through whole seed samples. The characterization of the pure GM is on a par with the existing reports. The purified GM inhibited ⊍-amylase and ⊍-glucosidase enzymes in vitro, with an IC50 of 21.08 ± 0.085 and 67.17 ± 5.15 ∼g mL−1 , respectively. CONCLUSION: Selective extraction prompts enhancement in the recovery of the bioactive compound, minimal use of resources, and promotes industrial viability. Characterization of the compound confirms the structure. Its enzyme inhibitory property makes GM a valuable compound in diabetic prevention/treatment. © 2021 Society of Chemical Industry.

Item Type:	Article
Uncontrolled Keywords:	Galactomannan; husk; dietary fibre; soluble fibre; characterization
Subjects:	600 Technology > 08 Food technology > 18 Processed foods > 01 Dietary Fiber 600 Technology > 08 Food technology > 30 Spices/Condiments > 08 Fenugreek
Divisions:	Plantation Products Spices and Flavour Technology
Depositing User:	Somashekar K S
Date Deposited:	18 Dec 2025 09:41
Last Modified:	18 Dec 2025 09:41
URI:	http://ir.cftri.res.in/id/eprint/20242

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