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Production of 2-hydroxy-4-methoxybenzaldehyde in roots of tissue culture raised and acclimatized plants of Decalepis hamiltonii Wight & Arn., an endangered shrub endemic to Southern India and evaluation of its performance vis-a-vis plants from natural habitat.
Giridhar, P. and Rajasekaran, T. and Nagarajan, S. and Ravishankar, G. A. (2004) Production of 2-hydroxy-4-methoxybenzaldehyde in roots of tissue culture raised and acclimatized plants of Decalepis hamiltonii Wight & Arn., an endangered shrub endemic to Southern India and evaluation of its performance vis-a-vis plants from natural habitat. Indian Journal of Experimental Biology, 42 (1). pp. 106-10. ISSN 0019-5189
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Abstract
Axillary buds obtained from field grown plants of D. hamiltonii were used to initiate multiple shoots on Murashige and Skoog's medium (MS) supplemented with 2 mg L(-1) 6-benzyl aminopurine (BA) and 0.5 mg L(-1) indole-3-acetic acid (IAA). Profuse rooting was achieved when the actively growing shoots were cultured on MS medium supplemented with 1.0 mg l(-1) indole-3-butyric acid (IBA). Regenerated plants were grown successfully in the plains, in contrast to wild growth in high altitudes and rocky crevices of hilly regions. Roots of different sizes from one-year-old tissue culture raised field grown plants had the same profile of 2-hydroxy-4-methoxybenzaldehyde as that of wild plants. A maximum of 0.14% and 0.12% 2-hydroxy-4-methoxybenzaldehyde was produced in roots of one year old tissue culture derived plants and greenhouse grown plants respectively.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | decalepis; in vitro shoots; tuberous roots; flavour compound |
| Subjects: | 500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs 600 Technology > 08 Food technology > 15 Flavour/Fragrance/Perfumes |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Users 197 not found. |
| Date Deposited: | 17 Aug 2011 09:09 |
| Last Modified: | 17 Aug 2011 09:09 |
| URI: | http://ir.cftri.res.in/id/eprint/2027 |
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