Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by cetyltrimethylammonium bromide.

Joshi, M. S. and Gowda, L. R. and Bhat, S. G. (1987) Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by cetyltrimethylammonium bromide. Biotechnology Letters, 9 (8). pp. 549-554. ISSN 0141-5492

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Abstract

Whole cells of lactose-fermenting K. fragilis had very low beta-galactosidase activity. Treating these cells with a cationic detergent, cetyltrimethylammonium bromide (0.1%), at 4 degree C for 5 min increased enzyme activity 480-fold. Detergent-treated cells readily hydrolysed lactose in milk and sweet whey; glucose produced was not metabolized further. Detergent-permeabilized cells could be used, e.g. to produce low lactose milk, or in utilization of whey. [From En summ.]

Item Type:	Article
Uncontrolled Keywords:	YEASTS-; whey, lactose Kluyveromyces detergent permeabilized hydrolysis in; DETERGENTS-; LACTOSE-; PERMEABILITY-; HYDROLYSIS-; WHEY-; lactose Kluyveromyces detergent permeabilized hydrolysis in whey
Subjects:	600 Technology > 08 Food technology > 27 Dairy products 600 Technology > 08 Food technology > 29 Microbiological food > 04 Yeast
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Jul 2008 11:23
Last Modified:	01 Oct 2018 09:24
URI:	http://ir.cftri.res.in/id/eprint/2031

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