Preparation of stearoyl lactic acid ester catalyzed by lipases from Rhizomucor miehei and porcine pancreas optimization using response surface methodology

Kiran, K. R. and Karanth, N. G. and Divakar, S. (1999) Preparation of stearoyl lactic acid ester catalyzed by lipases from Rhizomucor miehei and porcine pancreas optimization using response surface methodology. Applied Microbiology and Biotechnology, 52. pp. 579-584.

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Abstract

<p align="justify">The esterification reaction between stearic acid and lactic acid using Rhizomucor miehei lipase and porcine pancreas lipase was optimized for maximum esterification using response surface methodology. The formation of the ester was found to depend on three parameters namely enzyme/substrate ratio, lactic acid (stearic acid) concentration and incubation period. The maximum esteri®cation predicted by theoretical equa- tions for both lipases matched well with the observed experimental values. In the case of R. miehei lipase, stearoyl lactic acid ester formation was found to increase with incubation period and lactic acid (stearic acid) concentrations with maximum esteri®cation of 26.9% at an enzyme/substrate (E/S) ratio of 125 g mol)1. In the case of porcine pancreas lipase, esteri®cation showed a steady increase with increase in incubation period and lactic acid (stearic acid) concentration independent of the E/S ratios employed. In the case of PPL, a maximum esteri®cation of 18.9% was observed at an E/S ratio of 25 g mol)1 at a lactic acid (stearic acid) concentration of 0.09 M after an incubation period of 72 h.</p>

Item Type:	Article
Uncontrolled Keywords:	stearoyl lactic acid ester Rhizomucor miehei response surface methodology porcine pancreas
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	21 Nov 2005
Last Modified:	28 Dec 2011 09:25
URI:	http://ir.cftri.res.in/id/eprint/223

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