Bioassay of piperazine and some plant products with earthworms.

A bioassay method for estimating piperazine
citrate and other vermicidal agents has been
reported. Application of the bioassay method,
based on the response of earthworms (Pheretima
sp,) to the drugs in Ringer.Locke medium for
rapid screening of anthelmintic substances has
been demonstrated. Quantitative assessment
of different plant drugs for their vermicidal
potencies has been carried out and their relative
efftcacies with reference to piperazine citrate have
been studied. Piperazine coefficients of papaya
seed, mustard seed, garlic and clove show that
clove and garlic are superior to piperazine citrate
with respect to vermicidal potency.