14-Aminotetradecanoic acid exhibits antioxidant activity and ameliorates xenobiotics-induced cytotoxicity

Natural compounds with free-radical scavenging
activity have potential role in maintaining human health and
preventing diseases. In this study, we report the antioxidant
and cytoprotective properties of 14-aminotetradecanoic acid
(ATDA) isolated from the Decalepis hamiltonii roots.
ATDA is a potent scavenger of superoxide (O2
•-), hydroxyl
(•OH), nitric oxide (•NO), and lipid peroxide (LOO•) physiologically
relevant free radicals with IC50 values in nM
(36–323) range. ATDA also exhibits concentration-dependent
secondary antioxidant activities like reducing power,
metal-chelating activity, and inhibition of protein carbonylation.
Further, ATDA at nM concentration prevented
CuSO4-induced human LDL oxidation. ATDA demonstrated
cytoprotective activity in primary hepatocytes and
Ehrlich ascites tumor cells against oxidative stress inducing
xenobiotics apart from the in vitro free-radical scavenging
activity. The mechanism of cytoprotective action involved
maintaining the intracellular glutathione, scavenging of
reactive oxygen species, and inhibition of lipid peroxidation.
It is suggested that ATDA is a novel bioactive molecule with
potential health implications.