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Physicochemical studies on two forms of Bengal gram lipoxygenase: Implication for structural differences.
Borthakur, A. and Ramdoss, C. S. and Appu Rao, A. G. (1988) Physicochemical studies on two forms of Bengal gram lipoxygenase: Implication for structural differences. Biochimica et Biophysica Acta, 958. pp. 40-51.
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Biochimica_et_Biophysica_Acta_(BBA)_-_Lipids_and_Lipid_Metabolism,_Volume_958,_Issue_1,_19_January_1988,_Pages_40-51.pdf Restricted to Registered users only Download (1MB) |
Abstract
Two forms of lipoxygenase have been isolated from Bengal gram (Cicer arieiinum) and purified to apparent homogeneity. In sedimentation velocity experiments both forms showed a single symmetrical peak with an s20,W value of 5.5 f 0.5 S. From the SDS-polyacrylamide gel electrophoresis and gel filtration data they appeared to consist of a single polypeptide chain of identical size (M, = 95 000). The amino acid composition was essentially identical except for the proline and isoleucine contents. Kinetically, the two forms were quite distinct. The CD spectra of the two forms in the far-ultraviolet region were rather similar. However, in the near-ultraviolet region there were discernible differences. Although the antibody raised against each of them showed antigenic cross-reactivity, there was a perceptible difference in the cross-reaction with enzymes from other legumes. The results imply elements of dissimilarity in the structure of these two forms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Lipoxygenase; Protein structure; Circular dichroism; (Bengal gram seed) |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 22 Legumes-Pulses |
| Divisions: | Protein Chemistry and Technology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 02 Jun 2011 06:00 |
| Last Modified: | 28 Dec 2011 09:48 |
| URI: | http://ir.cftri.res.in/id/eprint/4852 |
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