Structural features of two amyloids from the hemicellulosic fraction of field bean (Dolichos lablab) hulls.

Salimath, P. V. and Tharanathan, R. N. (1982) Structural features of two amyloids from the hemicellulosic fraction of field bean (Dolichos lablab) hulls. Carbohydrate Research, 107. pp. 103-109.

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Abstract

Two amyloid-type fractions were isolated from field-bean (Dok~zos Zablab) hdls by 10 % alkali extraction followed by acetylation and solvent fractionation. The major, chloroform-insoluble fraction and a minor, chloroform-soluble fraction were found to be homogeneous in sedimentation analysis and molecular-sieve chromatography_ The polysaccharides contained xylose and glucose in various proportions_ Methylation analysis, periodate oxidation, Smith degradation, oxidation by chromium trioxide, and oligosaccharide studies indicated a new type of structure for the major fraction (glucose:xylose ratio of 1.9 : 1) in that it had a backbone of (144)-linked &D-glucose residues interspersed with single or multiple residues of (1+4)-linked b-D-xylose, and to which some single D-xylosyl groups are attached through O-6 of D-glucose. In contrast, the minor fraction (glucose: xylose ratio of 1 : 3.7) had a backbone of (l-+4)-linked p-D-xylose interspersed with (1-4)~/?-D-glucose and having a side chain of D-xylose, attached through O-6 of D-glucose. The third fraction was found to be a mixture of linear (1+4)-D-glucan and (1+4)-o-xylan.

Item Type:	Article
Uncontrolled Keywords:	field-bean, amyloid-type fractions, structural features
Subjects:	600 Technology > 08 Food technology > 22 Legumes-Pulses 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Jun 2011 09:35
Last Modified:	28 Dec 2011 09:50
URI:	http://ir.cftri.res.in/id/eprint/5526

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