Studies on groundnut proteins. I. Effect of sodium dodecyl sulphate on the physico-chemical properties.

Setty, K. J. and Rao, M. S. N. (1973) Studies on groundnut proteins. I. Effect of sodium dodecyl sulphate on the physico-chemical properties. Indian Journal of Biochemistry and Biophysics, 10 (3). 149-154, 29 ref..

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Abstract

Effect of addition of sodium dodecyl sulphate (SDS) of the physico-chemical properties of groundnut protein at pH 9.5 in glycine-NaOH buffer was studied. Gel filtration, ultracentrifugation and polyacrylamide gel electrophoresis data indicated that SDS caused dissociation of the proteins. Arachin dissociated to molecules approx. one-sixth its mol. wt. Canarachins did not appear to dissociate. In gel electrophoresis, it was observed that the dissociative effect of SDS was greater on the arachin 'monomer' than on the 'dimer'. Specific viscosity increased on addition of SDS. Difference spectra with troughs at 277-278 nm and 287-288 nm were observed in the presence of SDS. The intensity of these troughs increased with SDS concn. The binding of SDS by the protein gave an anomalous Scatchard plot.

Item Type:	Article
Uncontrolled Keywords:	GROUNDNUTS-; sodium dodecyl sulphate & dissociation of groundnut proteins; PROTEINS-(VEGETABLE); groundnut proteins, sodium dodecyl sulphate & dissociation of
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 19 Lipids-oils/fats > 05 Nuts
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 Sep 2013 04:59
Last Modified:	19 Sep 2013 04:59
URI:	http://ir.cftri.res.in/id/eprint/7107

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