Efficient and simple extraction of mouldy bran in a pulsed column extractor for recovery of amyloglucosidase in concentrated form.

Ghildyal, N. P. and Ramakrishna, M. and Lonsane, B. K. and Karanth, N. G. (1991) Efficient and simple extraction of mouldy bran in a pulsed column extractor for recovery of amyloglucosidase in concentrated form. Process Biochemistry, 26 (4). 235-241, 18 ref..

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Abstract

The recovery of amyloglucosidase [EC 3.2.1.3] from mouldy bran obtained from a solid-state fermentation system in a pulsed plug flow extractor was 86% with the use of 10 ml min-1 feed rate of the solvent and 87 min extraction time. The resulting extract vol. was twice that of the wt. of dry mouldy bran. An increase in extraction time at the same feed rate resulted in improved extraction efficiencies, but the resulting extract was too dilute. The use of a submerged column extractor for preventing possible channelling, together with the addition of calcium acetate to the feed solvent to reduce the viscosity of the extract, resulted in lower recoveries. Data indicate practical and economic advantages of pulsed plug flow extractors over the currently used percolation and multiple-contact counter-current leaching techniques.

Item Type:	Article
Uncontrolled Keywords:	AMYLASES-; BIOTECHNOLOGY-; BRAN-; CEREAL-PRODUCTS; DOWNSTREAM-PROCESSING; ENZYMES-; EXTRACTION-; WASTES-; GLUCAN-1,4-alpha-GLUCOSIDASES; GLUCOSIDASES-
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Jun 2011 06:19
Last Modified:	28 Dec 2011 10:01
URI:	http://ir.cftri.res.in/id/eprint/7994

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