Structural characterisation and determination of prebiotic activity of purified xylo-oligosaccharides obtained from Bengal gram husk (Cicer arietinum L.) and wheat bran (Triticum aestivum)

Madhukumar, M. S. and Muralikrishna, G. (2010) Structural characterisation and determination of prebiotic activity of purified xylo-oligosaccharides obtained from Bengal gram husk (Cicer arietinum L.) and wheat bran (Triticum aestivum). Food Chemistry, 118 (2). pp. 215-223. ISSN 0308-8146 (In Press)

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Abstract

Water extractable polysaccharides (WEPs) were isolated from Bengal gram husk and wheat bran. These WEP were subjected to driselase enzyme hydrolysis to obtain oligosaccharide mixtures, which were purified successively on Biogel P-2 and high performance liquid chromatographies. The molecular weight and structural features of the purified oligosaccharides were deduced using ESI-MS and 1H NMR, respectively. The prebiotic properties of these purified oligosaccharides were studied by using Bifidobacterium adolescentis NDRI 236. Increase in dry cell mass (0.7-0.9mg/ml) and decrease in pH (<5.8) due to production of short chain fatty acid (SCFA) indicated oligosaccharide fermentation. Acetate was the chief SCFA produced and its amount varied from 97.2% to 100%. The activities of xylanase (257-470mU/ml), xylopyranosidase (53-60mU/ml) and arabinofuranosidase (60-70mU/ml) in the culture broth indicated the breakdown of xylo-oligosaccharides and their subsequent utilisation by the bacterium for its growth.

Item Type:	Article
Uncontrolled Keywords:	Water extractable polysaccharides Bengal gram husk Wheat bran Xylo-oligosaccharides Prebiotic activity
Subjects:	600 Technology > 08 Food technology > 22 Legumes-Pulses 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 28 Polysaccharide Chemistry
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	15 Jun 2009 07:12
Last Modified:	28 Dec 2011 10:09
URI:	http://ir.cftri.res.in/id/eprint/9050

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