Reverse Micellar Extraction Coupled Adsorption for Purification of Bromelain

Surya Prakash, P. (2010) Reverse Micellar Extraction Coupled Adsorption for Purification of Bromelain. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	This work focuses on the purification of Bromelain enzyme using Reverse micellar system coupled with Adsorption. Reverse micellar system of CTAB/Iso‐octane/Hexanol/Butanol are used for the extraction and primary purification of Bromelain from crude aqueous extract of pineapple waste (core).The final aqueous solution with maximum purity fold (5.2) from the reverse micellar system is subjected to Adsorption using strongly acidic cation exchange resin at pH of 4.0.The optimized conditions for the adsorption using Amberlite IRA 120 sodium form ion exchange resin yield a maximum purity fold (10.0) of Bromelain.
Uncontrolled Keywords:	Bromelain enzyme; Reverse micellar system
Subjects:	600 Technology > 08 Food technology > 07 Food Engineering 600 Technology > 08 Food technology > 01 Analysis 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 Aug 2010 07:24
Last Modified:	17 Oct 2018 10:41
URI:	http://ir.cftri.res.in/id/eprint/9604

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