Identification and characterization of the n-6 fatty acid-producing Mucor rouxii native isolate CFR-G15

Mamatha, S. S. and Prakash, M. Halami and Venkateswaran, G. (2010) Identification and characterization of the n-6 fatty acid-producing Mucor rouxii native isolate CFR-G15. European Journal of Lipid Science and Technology, 112. pp. 380-389.

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Abstract

In zygomycetes fungi, many Mucor spp. have been known to produce g-linolenic acid (GLA) in their biomass. Among 250 soil samples screened, 20 Mucor isolates showed GLA in their mycelial mass under normal cultivation conditions. Sudan Black B was used for screening their qualitative oleaginesity. Among the representative isolates, Mucor sp. CFR-G15, when grown in a fat-producing medium, showed a maximum lipid content of 30 6 1.32% in its mycelia and 14.42 6 0.74% GLA. By using gene-specific primers, the 18S rRNA gene and the D6 DES gene were amplified by PCR technique. The nucleotide sequences of the 18S rRNA and D6 DES genes exhibited .98% homology with M. rouxii ATCC 24905 (accession nos. AF117923 and AF296076, respectively), suggesting taxonomic identity. The native isolate M. rouxii CFR-G15 reported in this study was found to be promising for the development of an economical process in the industrial production of GLA.

Item Type:	Article
Uncontrolled Keywords:	Desaturase enzyme g-Linolenic acid Mucor rouxii Phylogeny PUFA
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	14 Sep 2010 06:19
Last Modified:	16 May 2012 08:47
URI:	http://ir.cftri.res.in/id/eprint/9656

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