Biotransformation of Digitoxin in Cell Cultures of Capsicum frutescens in the Presence of beta-cyclodextrin

Ramachandra Rao, S. and Usha, Tripathi and Ravishankar, G. A. (2002) Biotransformation of Digitoxin in Cell Cultures of Capsicum frutescens in the Presence of beta-cyclodextrin. Biocatalysis and Biotransformation, 20 (2). pp. 137-143.

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Abstract

Cell suspension cultures of Capsicum frutescens accumulated digoxin, purpureaglycoside A and other unknown derivatives when digitoxin, a cardiac glycoside, was used as a precursor. The feeding of digitoxin complexed with b-cyclodextrin increased the accumulation of digoxin, purpureaglycoside A and other unknown derivatives. Control cultures (without digitoxin) did not produce any of these metabolites. The growth of cells was affected by both digitoxin as well as digitoxin-b-cyclodextrin. The accumulation of purpureaglycoside A and digoxin reached a maximum of 1241 and 374mg 100 ml21 culture on the 6th and 2nd day, respectively, which was 3.9 and 4.5 fold higher than cultures treated with digitoxin alone (sampled on the 13th day). The other unknown derivatives formed in digitoxin-b-cyclodextrin fed cultures were 15 times higher than digitoxin alone fed C. frutescens cultures. The addition of glucose to digitoxin-b-cyclodextrin treated cultures increased the accumulation of purpureaglycoside A which reached a maximum of 3589mg 100 ml21 culture after 12 h incubation, which was a 2.9 fold increase over cultures treated with digitoxin-b-cyclodextrin alone.

Item Type:	Article
Uncontrolled Keywords:	Biotransformation; b-cyclodextrin; Digitoxin; Digoxin; Purpureaglycoside A; Capsicum frutescens
Subjects:	600 Technology > 08 Food technology > 23 Vegetables 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	24 Sep 2010 09:52
Last Modified:	28 Dec 2011 10:18
URI:	http://ir.cftri.res.in/id/eprint/9695

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