Nakkeeran, E. and Umesh Kumar, S. and Subramanian, R. (2009) Process Assessment for the Purification of Aspergillus carbonarius Polygalacturonase Produced by Submerged and Solid-State Fermentations. International Journal of Engineering and Technology, 1 (4). pp. 278-281.
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Abstract
Abstract—Integrated membrane process (IMP) and alginate
affinity precipitation (AAP) were assessed for the purification
of Aspergillus carbonarius polygalacturonase (PG) obtained
after submerged (SmF) and solid-state fermentations (SSF).
IMP enhanced the purity (4.69 fold) and recovery (76%) of
SmF-PG, but did not improve the purity of SSF and commercial
PG due to the presence of other similar molecular mass proteins.
AAP enhanced the PG purity and recovery in all the PG
samples (SmF, SSF and commercial PG). IMP offers several
process advantages over AAP but not suitable for purification
of all types of PGs. Our studies on SmF- and SSF-PG had shown
that downstream process needs are specific for individual cases
and warrants careful considerations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Alginate affinity precipitation, integrated membrane process, polygalacturonase, submerged and solid-state fermentations. |
| Subjects: | 600 Technology > 05 Chemical engineering > 04 Fermentation Technology 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology |
| Divisions: | Food Microbiology Food Engineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 24 Feb 2011 06:52 |
| Last Modified: | 28 Dec 2011 10:21 |
| URI: | http://ir.cftri.res.in/id/eprint/9929 |
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