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Supercritical CO2 extraction of Mortierella alpina single cell oil: Comparison with organic solvent extraction.
Nisha, A. and Udaya Sankar, K. and Venkateswaran, G. (2012) Supercritical CO2 extraction of Mortierella alpina single cell oil: Comparison with organic solvent extraction. Food Chemistry, 133 . pp. 220-226.
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Food Chemistry, Volume 133, Issue 1, 1 July 2012, Pages 220-226.pdf - Published Version Restricted to Registered users only Download (665kB) | Request a copy |
Abstract
Analysis of supercritical fluid extracted (SFE) and organic solvent extracted lipids from freeze dried Mortierella alpina biomass was carried out. The effect of biomass pre-treatment on effectual lipid recovery was also investigated. Results indicated that the methods employed viz., SFE and organic solvent (Sox Tec™) exhibited significant variation in lipid extraction efficacy. Efficacy of SFE on lipid recovery (38.73%) was significantly lower when compared to that of Sox Tec™ method (51.47%) because the organic solvent can also extract polar lipids. Biomass pretreatment by enzymatic method resulted in a substantial increase in lipid recovery. Maximum disruption of cell wall evidenced by scanning electron microscopy of treated cells warranted their higher mass transfer and efficient extraction. Characterisation of the extracted lipids by GC and GC–MS revealed considerable variation in the fatty acid profile. The FTIR spectrum indicated a prominent cis-stretching vibration, no evident trans- stretching vibration and no major discrepancies between the SFE and Sox Tec™ extracts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Mortierella, Supercritical extraction, Sox Tec™ extraction Total lipids |
| Subjects: | 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 600 Technology > 08 Food technology > 01 Analysis |
| Divisions: | Food Engineering Food Microbiology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 23 Feb 2012 05:51 |
| Last Modified: | 28 Dec 2016 13:00 |
| URI: | http://ir.cftri.res.in/id/eprint/10655 |
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