Extracellular phytase from Aspergillus niger CFR 335: purification and characterization.

Gunashree, B. S. and Venkateswaran, G. (2015) Extracellular phytase from Aspergillus niger CFR 335: purification and characterization. Journal of Food Science and Technology, 52 (7). pp. 4558-4564.

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Abstract

Phytase, that is extensively used as a feed additive is capable of hydrolyzing phytic acid, an antinutrient found in about 60–80 % of all the plant commodities. This enzyme improves the bioavailability of essential minerals such as Ca2+, Mg2+, P, Zn2+, Fe3+, that are bound to phytic acid. An extracellular phytase from a local fungal isolate, Aspergillus niger CFR 335 was purified to homogeneity through a threestep column chromatography using DEAE-Sephadex anion exchanger. An active fraction of the enzyme was obtained with NaCl gradient of 2.5Min DEAE Sephadex column. The enzyme was purified up to 16 fold with a yield of 28.5 %. Substrate specificity studies revealed a highest specific activity of 32.6±3.1 U/mg for sodium phytate with the Km value of 0.08±0.1 mM. The molecular weight of the enzyme was 66 kDa with an optimum temperature of 30 °C and pH 4.5. Up to 80 % of the activity was retained even after storing the enzyme for 6 months at 4 °C.

Item Type:	Article
Uncontrolled Keywords:	Extracellular phytase . Phytic acid .Monogastrics . Aspergillus niger CFR 335 . Purification
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	11 Dec 2015 07:04
Last Modified:	11 Dec 2015 07:04
URI:	http://ir.cftri.res.in/id/eprint/12026

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