Lipase Inhibitors from Nigella Sativa and Punica Granatum as an Effective Approach Towards Controlling Obesity.

Shamsiya, T. K. and Manjunatha, J. R. and Manonmani, H. K. (2016) Lipase Inhibitors from Nigella Sativa and Punica Granatum as an Effective Approach Towards Controlling Obesity. LIFE: International Journal of Health and Life-Sciences, 2 (2). pp. 1-19.

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Abstract

Two distinct lipase inhibitor cocktails RAYstat4ns and SHAMstat3pg have been purified from seeds of Nigella sativa and Punica granatum respectively. Structural characterization has shown RAYstat4ns to be a mixture of 3 polyunsaturated fatty acids and one saturated fatty acid while SHAMstat3pg is a triglyceride mixture comprising of three different kinds of polyunsaturated fatty acids. Ethanol extracted fractions of seeds of Nigella saliva and Punic a granatum having high lipase inhibitory activity were purified by HPLC followed by TLC to yield respectively RAYstat4ns and SHAMstat3pg . These plant based lipase inhibitors have shown good lipase inhibitory activity against pancreatic lipase with mixed type of inhibition. RAYstat4ns and SHAMstat3pg have also shown inhibition of hormone sensitive lipase. The experimental IC50 of the purified lipase inhibitors RAYstat4ns and SHAMstat3pg was found to be respectively 4.02μg/μl and 7.35 μg/μl for pancreatic lipase and 6.31 μg/μl and 11.45 μg/μl respectively for hormone sensitive lipase. Hence ,these plant-based lipase inhibitors can be used in controlling lipolysis and insulin resistance in addition to inhibiting absorption of dietary lipid into the living system. In vivo studies could help confirm the potency of these plant based isolates as efficient lipase inhibitors.

Item Type:	Article
Uncontrolled Keywords:	Lipase inhibitor, Nigella saliva, Punica granatum, pancreatic lipase inhibitor, hormone sensitive lipase inhibitor, anti-obesity
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 24 Fruits
Divisions:	Food Protectants and Infestation Control Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	18 Jan 2018 06:30
Last Modified:	18 Jan 2018 06:30
URI:	http://ir.cftri.res.in/id/eprint/13303

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