Strain Improvement Through Mutagenesis and Optimization of Protease Production By Aspergillus Terreus Cjs-127 using Jatropha Seed Cake as Substrate.

Jyothna, B. and Somashekar, D. (2017) Strain Improvement Through Mutagenesis and Optimization of Protease Production By Aspergillus Terreus Cjs-127 using Jatropha Seed Cake as Substrate. Journal of Microbiology Biotechnology and Food Sciences, 7 (2). pp. 174-180.

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Abstract

Jatropha curcas seedcake (JSC), a byproduct of the biodiesel industry was utilized for the production of protease. To enhance the protease production, the parent strain of Aspergillus terreus CJS-127 subjected to UV (15, 30, 60, 90, 120 and 150 min) and chemical mutagenesis by using ethyl methanesulphonate (EMS-125, 250 and 500 mg). Two mutant strains designated as UV-6 and EMS-5 selected for optimization of protease production using JSC medium by submerged fermentation. Under the optimized conditions of pH 7.0, temperature 30°C, incubation period 72 h, inoculum 105/ml spores, 150 rpm agitation, JSC at 2% level, the maximum protease production was 172.32 and 163.57 U/ml for EMS-5 and UV-6 mutant strains respectively. The addition of maltose (2%) was found to enhance the protease production in the mutant strains. The protease activity increased by 2.30 and 2.41 folds for UV 6 and EMS 5 mutant strains after optimization of cultural conditions.

Item Type:	Article
Uncontrolled Keywords:	Strain improvement, Jatropha seedcake, Protease, Aspergillus terreus CJS-127, mutagenesis
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	19 Jan 2018 04:20
Last Modified:	19 Jan 2018 04:20
URI:	http://ir.cftri.res.in/id/eprint/13325

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