Synthesis of ethyl isovalerate using Rhizomucor miehei lipase: optimization.

Chowdary, G. V. and Prapulla, S. G. (2003) Synthesis of ethyl isovalerate using Rhizomucor miehei lipase: optimization. Applied Biochemistry and Microbiology, 39 (3). pp. 278-83.

PDF Applied Biochemistry and Microbiology, Vol. 39, No. 3, 2003, pp. 243–248.pdf - Published Version Restricted to Registered users only Download (43kB) | Request a copy

Abstract

Immobilized lipase from Rhizomucor miehei (Lipozyme IM-20) was used to catalyze the esterification reaction between isovaleric acid and ethanol to synthesize ethyl isovalerate in n-hexane. Response surface methodology based on five-level four-variable central composite rotatable design was employed to optimize four important reaction variables such as enzyme/substrate E/S ratio, substrate concentration, incubation time, and temperature affecting the synthesis of ethyl isovalerate. The optimum conditions predicted for achieving maximum ester yield (500 mM) are as follows: E/S ratio, 48.41 g/mol; substrate concentration, 1 M; reaction time, 60 h; temperature, 60 degrees C. The predicted value matched well with experimentally obtained value of 487 mM.

Item Type:	Article
Uncontrolled Keywords:	Rhizomucor miehei, Lipozyme IM-20, ethyl isovalerate, ester synthesis
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Mar 2018 08:09
Last Modified:	22 Mar 2018 09:22
URI:	http://ir.cftri.res.in/id/eprint/2070

Actions (login required)

View Item