Microscopic observation of Sudan black B staining to monitor lipid production by microbes.

Thakur, M. S. and Prapulla, S. G. and Karanth, N. G. (1988) Microscopic observation of Sudan black B staining to monitor lipid production by microbes. Journal of Chemical Technology and Biotechnology, 42 (2). pp. 129-134.

PDF J . Chem. Tech. Biotechnol. 1988, 42, 129-134.pdf - Published Version Restricted to Registered users only Download (336kB)

Abstract

In experimental investigations on the improvement of yields and productivities of lipids from oleaginous micro-organisms in fermenters, a bottleneck is the speedy determination of the extent of accumulation of the intracellular lipids. The use of Sudan Black B for staining and direct observation under the microscope enables the rapid observation of the qualitative status of lipid production in the cells. A change in the colour of the stain @om dark to light blue observed during the course of the fermentation has been found to be related to a change in the extent of unsaturation of the lipids.

Item Type:	Article
Uncontrolled Keywords:	Microbial lipids, Rhodotorula gracilis, fermentation, oil globules, intracellular lipids, saturation, microscopic observation
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 22 Lipid Chemistry 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 May 2016 07:33
Last Modified:	26 May 2016 07:33
URI:	http://ir.cftri.res.in/id/eprint/4639

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