Successive cultivation of selected cellulolytic fungi on rice straw and wheat bran for economic production of cellulases and D-xylanase.

Shamala, T. R. and Sreekantiah, K. R. (1987) Successive cultivation of selected cellulolytic fungi on rice straw and wheat bran for economic production of cellulases and D-xylanase. Enzyme and Microbial Technology, 9 (2). pp. 97-101.

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Abstract

Production of cellulase [1,4-(1,3;1,4)-fl-o-glucan 4-glucanohydrolase, EC 3.2.1.4], fl-D-glucosidase (fl-Dglucoside glucanohydrolase, EC 3.2.1.21) and o-xylanase (1,4-fl-D-xylan xylanohydrolase, EC 3.2.1.8) by successive cultivation of Aspergillus ustus, Trichoderma sp. (a), Trichoderma sp. (b), Botrytis sp. and Sporotrichum pulverulentum on rice straw (RS) and wheat bran (WB) substrates by solid state fermentation and concurrent degradation of the substrates were studied. For every gram of dry RS used, it was possible to obtain 14 U filter paper activity (FPA), 22 U carboxymethylcellulase (CMCase), 48 U fl-o-glucosidase and 1431 U D-xylanse, after recycling the same substrate five times. This also resulted in 55% loss, by weight of the substrate, 64% in fibre, 32% in lignin and 46% in minerals. WB under similar conditions of successive cultivation could yield 8 U FPA, 19 U CMCase, 81 U fl-D-glucosidase and 788 U D-xylanase. Concurrently, the total loss in substrate was 73%, the decrease in fibre was 52%, lignin 28% and minerals 86%. Amount of crude protein in the residual RS and WB was 7.2 and 10.3% respectively. The data indicate that a single fungal isolate could be grown on a substrate for five successive cycles but that the enzyme yields were less than when different strains were employed.

Item Type:	Article
Uncontrolled Keywords:	Fungal enzymes; solid state fermentation; cellulase; fl-D-glucosidase; D-xylanase; rice straw; wheat bran; fibre; lignin; ash; crude protein
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Jun 2011 10:50
Last Modified:	28 Dec 2011 09:51
URI:	http://ir.cftri.res.in/id/eprint/5887

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