An Enzyme Immunoassay for the Organochlorine Insecticide Hexachlorocyclohexane (HCH), Through Conversion to Trichlorophenols

Beasley, H. L. and Pasha, A. and Guihot, S. L. (2000) An Enzyme Immunoassay for the Organochlorine Insecticide Hexachlorocyclohexane (HCH), Through Conversion to Trichlorophenols. Food and Agricultural Immunology , 12. pp. 203-215.

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Abstract

A method for immunoassay analysis of the organochlorine insecticide, hexachlorocyclohexane (HCH) has been developed, based upon alkaline conversion in standards and samples to trichlorobenzenes. The trichlorobenzenes were detected through antisera developed to haptens containing either a trichlorobenzene or trichlorpyridine moiety, developed using the herbicides, 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) and triclopyr, respectively. An enzyme conjugate based on 2,4,5-trichlorophenol provided most sensitive and specific detection. Although the assays cross-reacted with the herbicides, they did not suffer from the major disadvantage of extremely strong cross-reaction with cyclodiene organochlorines reported for a commercial HCH assay. The most sensitive assay had a lower detection limit of 20 mg l–1 in drinking water and was applied to water and soil matrices.

Item Type:	Article
Uncontrolled Keywords:	HCH, lindane, trichlorobenzenes, conversion, ELISA
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry 600 Technology > 08 Food technology > 01 Analysis
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Sep 2010 09:40
Last Modified:	28 Dec 2011 10:18
URI:	http://ir.cftri.res.in/id/eprint/9702

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