Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates

Production of extracellular chitin deacetylase by
Colletotrichum lindemuthianum ATCC 56676 under solid
substrate fermentation was studied. The suitability of
shrimp shell chitin waste (SSCW) and commercial wheat
bran (CWB) was evaluated for maximal enzyme production.
CWB medium (pH 6.4±0.2) supplemented with
chitosan favoured maximal chitin deacetylase yield of
460.4±14.7 unit/g initial dry substrate (U/g IDS) at 96 h
as compared to maximal yield of 392.0±6.4 U/g IDS at
192 h in SSCW medium (pH 8.7±0.2) at 25 °C incubation
temperature and 60% (w/w) initial moisture content of
medium. Along with chitin deacetylase, C. lindemuthianum
ATCC 56676 produced maximum endo-chitinase (0.28±
0.03 U/g IDS at 144 h) and β-N-acetylhexosaminidase
(0.79±0.009 U/g IDS at 192 h) in CWB medium and 0.49±
0.05 U/g IDS of endo-chitinase at 264 h and 0.38±0.04 U/g
IDS of β-N-acetylhexosaminidase at 96 h of incubation in
SSCW medium. SEM studies indicated the difference in the
morphology of mycelia and hyphae of C. lindemuthianum
ATCC 56676 when grown on different solid substrates.
Production of chitin deacetylase by SSF is being reported
for the first time.