Inhibition of Pathogenic Cell Wall Degrading Enzymes and its Role in Pineapple Stem End Rot Disease Development.

Pooja, Marigoli (2011) Inhibition of Pathogenic Cell Wall Degrading Enzymes and its Role in Pineapple Stem End Rot Disease Development. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Student Project Report
Additional Information:	Pathogenic Ceratocystis paradoxa produces a mixture of cell wall degrading enzymes during pathogenesis in pineapple fruit. The tissue deterioration and maceration in the soft rot is mainly due to these cell wall hydrolyzing enzymes. Our study reveals that cellulases are the major group of enzymes the pathogen relies on for establishment and causing infection and bringing rapid changes of structural disintegration of the pineapple fruit tissue. Therefore standardization of Cellulase activity in Ceratocystis paradoxa was carried out. Inhibition of cellulase enzymes using different GRAS chemicals and preservatives were carried out. The results indicated that sodium benzoate, sodium carbonate, ellagic acid are potential chemicals for Inhibition of Ceratocystis paradoxa pathogenic cellulase enzymes. Thus, the result also provided alternate but safe chemicals with no pesticide residues that can used to control ‘Stem end rot’ disease development. The growth inhibition of pathogenic fungi by screened cellulase is a promising finding. As, many of these chemicals are approved for the usage in food preservation. Therefore by standardizing the appropriate concentration and the effect of fungal inhibition can be further improved and utilized for the formulation post harvest treatments of pineapples
Uncontrolled Keywords:	pineapple fruit, pathogenesis, Ceratocystis paradoxa,
Subjects:	600 Technology > 08 Food technology > 24 Fruits 500 Natural Sciences and Mathematics > 10 Plants > 03 Plant Pathology
Divisions:	Fruit and Vegetable Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Jul 2011 06:42
Last Modified:	28 Dec 2011 10:26
URI:	http://ir.cftri.res.in/id/eprint/10267

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