Lakshmi, M. C. and Madhusudhan, M. C. and Raghavarao, K. S. M. S. (2012) Extraction and Purification of Lipoxygenase from Soybean Using Aqueous Two-Phase System. Food and Bioprocess Technology, 5. pp. 193-199.
![[thumbnail of Food_and_Bioprocess_Technology_2012_5_1_193–199.pdf]](http://ir.cftri.res.in/style/images/fileicons/application_pdf.png "Food_and_Bioprocess_Technology_2012_5_1_193–199.pdf")
PDF
Food_and_Bioprocess_Technology_2012_5_1_193–199.pdf - Published Version
Restricted to Registered users only
Download (185kB)
Abstract
The purification of lipoxygenase in soybeans
(richest known source) was attempted by precipitation,
using polyethylene glycol, followed by aqueous two-phase
extraction (ATPE). The preliminary purification by
precipitation was standardized which resulted in two-fold
purification. The influence of process parameters such as
phase forming salt, molecular weight of phase forming
polymer, tie line length, and phase volume ratio on the
partitioning behavior of lipoxygenase during ATPE was
investigated. The aqueous two-phase system of polyethylene
glycol 6000 and ammonium sulfate, with inherent pH of 5.2,
was found to be most suitable. The tie line length of 41.25%
and volume ratio of 1.7 have resulted in 125% activity
recovery with the overall purification factor of 4.38 compared
to crude enzyme extract.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Lipoxygenase . Aqueous two-phase extraction . Precipitation . Purification |
| Subjects: | 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 22 Legumes-Pulses > 05 Soya bean 600 Technology > 08 Food technology > 05 Processing and Engineering |
| Divisions: | Food Engineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 11 Mar 2013 05:54 |
| Last Modified: | 11 Mar 2013 05:54 |
| URI: | http://ir.cftri.res.in/id/eprint/11141 |
Actions (login required)
- View Item